Chu, T.-H. | Search bioRxiv

bioRxiv2026

Reduced LANCL1-AS1 in old human skeletal muscle diminishes mitochondrial activity, shortens mt-mRNA poly(A) tails, and suppresses myogenesis

Regeneration of skeletal muscle preserves muscle mass and function, which decline with age. Here, we sought to identify long noncoding (lnc)RNAs involved in skeletal muscle myogenesis and potentially relevant to muscle aging. Cross-sectional analysis of skeletal muscle transcriptomes from healthy 22-through 89-year-old individuals revealed lncRNA LANCL1-AS1 among the top declining transcripts. Conversely, LANCL1-AS1 increased robustly during skeletal myogenesis and promoted myogenic differentiation in culture. Affinity pulldown by ChIRP followed by mass spectrometry revealed that LANCL1-AS1 associated with the mitochondrial protein LRPPRC, enhancing the formation of the chaperone complex LRPPRC-SLIRP, which maintains longer poly(A) tails of mitochondrial (mt-)mRNAs and stabilizes mt-mRNAs. Importantly, while myoblasts from old rhesus monkey muscle expressed lower levels of LANCL1-AS1 and mt-mRNAs, and displayed lower mitochondrial activity than young monkey myoblasts, overexpressing LANCL1-AS1 in old myoblasts restored mitochondrial activity and myogenesis. We propose that the age-associated reduction in LANCL1-AS1 contributes to impaired mitochondrial function and reduced myogenic capacity in aging skeletal muscle.

molecular biology↗

bioRxiv2025

Inferior Reinnervation of Reverse End-to-Side Nerve Transfer in a Delayed Nerve Repair Rat Model

ObjectiveReverse end-to-side (RETS) nerve transfer is a recent surgical technique to augment injured nerve function by supplying a dispensable donor motor nerve to the side of the distal injured nerve. Although clinical studies have suggested advantages of RETS transfer for upper extremity repairs, uncertainties remain regarding its underlying mechanism. Furthermore, our recent clinical studies using electrophysiological examinations revealed no contribution from the donor nerve. Given that most experimental studies were conducted on acutely injured nerves, our objective is to 1) reassess the effectiveness RETS nerve transfer in a rat model of chronic nerve injury and repair; 2) investigate the potential nerve-babysitting effect; and 3) investigate how availability of regenerating tracks, i.e. bands of Bungner, of recipient nerve affects donor nerve regeneration. MethodsObturator and femoral nerve were used as donor and recipient nerves, respectively. Electromyogram, retrograde labeling of regenerated motoneurons and neuromuscular junction (NMJ) formation were used to compare regenerative ability of donor nerve in acute and delayed RETS transfer where the femoral nerve in the latter group was injured by double ligations 8 weeks prior. Nerve-babysitting effect on injured nerve was investigated by 1) no intervention; 2) perineurial window creation; and 3) RETS transfer to femoral nerve in delayed repair model. The effects of availability of regeneration tracks were investigated by severing proximal femoral nerve, allowing complete denervation compared partial denervation in double ligations, followed by acute and delayed repairs. ResultsEMG and motoneuron quantification confirmed inferiority of donor nerve regeneration into recipient nerve in delayed RETS transfer compared to acute repair, yet donor axons reached target muscle and formed NMJs in both conditions. Same functional assessments revealed nerve baby-sitting effects did not significantly contribute to repair success but availability of regeneration tracks in the recipient nerve may influence the final outcomes. ConclusionsOur study offered insights into the effectiveness of RETS nerve transfer in clinically relevant settings, underscoring the compounded impact of delayed intervention and native nerve regeneration which both negatively affect the efficacy of RETS nerve transfer.

neuroscience↗