Biology subjects

Cheng, A. W.

Publications and source records attributed to Cheng, A. W..

2 recordsLinked to original sources

bioRxiv2018

Split Selectable Markers

Selectable markers are widely adopted in transgenesis and genome editing for selecting engineered cells with desired genotype but are limited in choices. We present here split selectable markers each allowing for selection of multiple "unlinked" transgenes in the context of lentivirus-mediated transgenesis as well as CRISPR/Cas-mediated biallelic knock-ins. Future development of split selectable markers may support enrichment or selection of "hyper-engineered" cells containing tens of transgenes or genetic modifications.

bioengineering↗

bioRxiv2018

CRISPR Artificial Splicing Factors

We report here the engineering of CRISPR Artificial Splicing Factors (CASFx) based on an RNA-targeting CRISPR/Cas system. We showed that simultaneous exon inclusion and exclusion can be induced at distinct targets by differential positioning of CASFx. We also created inducible CASFx (iCASFx) using the FKBP-FRB chemical-inducible dimerization domain, allowing small molecule control of alternative splicing.

bioengineering↗