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Biology subjects

Char, S.

Publications and source records attributed to Char, S..

2 recordsLinked to original sources

The Dayhoff Atlas: scaling sequence diversity for improved protein generation

Organized information powers modern biology, a framework pioneered by Margaret Dayhoffs Atlas of Protein Sequence and Structure and advanced by todays databases and computational methods. Here, we extend this paradigm for the AI era, presenting the Dayhoff Atlas of protein sequence data and generative models to accelerate protein biology and design. The Atlas introduces GigaRef, the largest open dataset of natural proteins, spanning 3.34B genomic and metagenomic sequences across 1.70B clusters, and BackboneRef, which distills structural information from 240,811 synthetic backbones into 46M synthetic sequences. Leveraging these datasets, we trained the Dayhoff protein language models, which can predict mutation effects, scaffold structural motifs, and generate novel proteins within families. Training on metagenomic and structure-based synthetic sequences increased the expression rates of generated proteins, demonstrating the value of data diversity and scale. We release the Dayhoff Atlas code, datasets, and models under a permissive license to empower computation in protein design.

bioengineering↗

ProtNote: a multimodal method for protein-function annotation

Understanding the protein sequence-function relationship is essential for advancing protein biology and engineering. However, fewer than 1% of known protein sequences have human-verified functions. While deep learning methods have demonstrated promise for protein function prediction, current models are limited to predicting only those functions on which they were trained. Here, we introduce ProtNote, a multimodal deep learning model that leverages free-form text to enable both supervised and zero-shot protein function prediction. ProtNote not only maintains near state-of-the-art performance for annotations in its train set, but also generalizes to unseen and novel functions in zero-shot test settings. We envision that ProtNote will enhance protein function discovery by enabling scientists to use free text inputs, without restriction to predefined labels - a necessary capability for navigating the dynamic landscape of protein biology.

bioengineering↗