Search bioRxiv⌕ Search

Biology subjects

Causey, J.

Publications and source records attributed to Causey, J..

3 recordsLinked to original sources
bioRxiv2019

Minor QTLs mining through the combination of GWAS and machine learning feature selection

IntroductionMinor QTLs mining has a very important role in genomic selection, pathway analysis and trait development in agricultural and biological research. Since most individual loci contribute little to complex trait variations, it remains a challenge for traditional statistical methods to identify minor QTLs with subtle phenotypic effects. Here we applied a new framework which combined the GWAS analysis and machine learning feature selection to explore new ways for the study of minor QTLs mining.\n\nResultsWe studied the soybean branching trait with the 2,137 accessions from soybean (Glycine max) diversity panel, which was sequenced by 50k SNP chips with 42,080 valid SNPs. First as a baseline study, we conducted the GWAS GAPIT analysis, and we found that only one SNP marker significantly associated with soybean branching was identified. We then combined the GWAS analysis and feature importance analysis with Random Forest score analysis and permutation analysis. Our analysis results showed that there are 36,077 features (SNPs) identified by Random Forest score analysis, and 2,098 features (SNPs) identified by permutation analysis. In total, there are 1,770 features (SNPs) confirmed by both of the Random Forest score analysis and the permutation analysis. Based on our analysis, 328 branching development related genes were identified. A further analysis on GO (gene ontology) term enrichment were applied on these 328 genes. And the gene location and gene expression of these identified genes were provided.\n\nConclusionsWe find that the combined analysis with GWAS and machine learning feature selection shows significant identification power for minor QTLs mining. The presented research results on minor QTLs mining will help understand the biological activities that lie between genotype and phenotype in terms of causal networks of interacting genes. This study will potentially contribute to effective genomic selection in plant breeding and help broaden the way of molecular breeding in plants.

bioinformatics↗
bioRxiv2019

Minor QTLs mining through the combination of GWAS and machine learning feature selection

IntroductionMinor QTLs mining has a very important role in genomic selection, pathway analysis and trait development in agricultural and biological research. Since most individual loci contribute little to complex trait variations, it remains a challenge for traditional statistical methods to identify minor QTLs with subtle phenotypic effects. Here we applied a new framework which combined the GWAS analysis and machine learning feature selection to explore new ways for the study of minor QTLs mining.\n\nResultsWe studied the soybean branching trait with the 2,137 accessions from soybean (Glycine max) diversity panel, which was sequenced by 50k SNP chips with 42,080 valid SNPs. First as a baseline study, we conducted the GWAS GAPIT analysis, and we found that only one SNP marker significantly associated with soybean branching was identified. We then combined the GWAS analysis and feature importance analysis with Random Forest score analysis and permutation analysis. Our analysis results showed that there are 36,077 features (SNPs) identified by Random Forest score analysis, and 2,098 features (SNPs) identified by permutation analysis. In total, there are 1,770 features (SNPs) confirmed by both of the Random Forest score analysis and the permutation analysis. Based on our analysis, 328 branching development related genes were identified. A further analysis on GO (gene ontology) term enrichment were applied on these 328 genes. And the gene location and gene expression of these identified genes were provided.\n\nConclusionsWe find that the combined analysis with GWAS and machine learning feature selection shows significant identification power for minor QTLs mining. The presented research results on minor QTLs mining will help understand the biological activities that lie between genotype and phenotype in terms of causal networks of interacting genes. This study will potentially contribute to effective genomic selection in plant breeding and help broaden the way of molecular breeding in plants.

bioinformatics↗
bioRxiv2019

CNNcon: A Quantitative Imaging Tool for Lung CT Image Feature Analysis

BackgroundLung CT scans are widely used for lung cancer screening and diagnosis. Current research focuses on quantitative analytics (radiomics) to improve screening and detection accuracy. However there are very limited numbers of portable software tools for automatic lung CT image analysis.\n\nResultsHere we build a Docker container, CNNcon, as a quantitative imaging tool for analyzing lung CT image features. CNNcon is developed from our recently published algorithm for nodule analysis, based on convolutional neural networks (CNN). When provided with a list of the centroid coordinates of regions of interest (ROI) in a volumetric CT study containing potential lung nodules, CNNcon can automatically generate highly accurate malignancy prediction of each ROI. CNNcon can also generate a vector of image features of each ROI, to facilitate further analyses by combining image features and other clinical features. As a Docker container, CNNcon is portable to various computer systems, convenient to install, and easy to use. CNNcon was tested on different computer systems and generated identical results.\n\nConclusionsWe anticipate that CNNcon will be a useful tool and broadly acceptable to the research community interested in quantitative image analysis.\n\nAvailabilityCNNcon and document are publicly available and can be downloaded from the website: http://bioinformatics.astate.edu/CNN-Container/

bioinformatics↗