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Castaneda-Sastre, E.

Publications and source records attributed to Castaneda-Sastre, E..

2 recordsLinked to original sources

Complete loss of lipoprotein biosynthesis machinery in an obligate intracellular bacterium

Orientia tsutsugamushi is a cytoplasm-dwelling, obligate intracellular, diderm bacterium with an unusual cell envelope. It lacks lipopolysaccharide and possesses only a minimal peptidoglycan-like cell wall. Here, we report that O. tsutsugamushi also lacks the two acyltransferase components of the lipoprotein biosynthesis pathway (Lgt, Lnt) and the entire lipoprotein transport machinery (Lol system). O. tsutsugamushi encodes several proteins that are conserved as lipoproteins among other organisms, including Pal and components of the beta-barrel assembly machine (BAM) complex, but these lack the canonical lipobox motif, consistent with the absence of lipidation pathways. Instead, we find that O. tsutsugamushi Pal compensates for the lack of lipidation by incorporating two predicted amphipathic helices, a feature not observed in Pal homologues from other organisms. These findings suggest that O. tsutsugamushi has compensated for a lack of lipoprotein assembly machinery through alternative mechanisms. We speculate on the possible evolutionary advantage of this highly unusual loss of the lipoprotein biosynthesis machinery in the context of an already minimal cell envelope.

microbiology↗

The Hok bacterial toxin: diversity, toxicity, distribution and genomic localization

The hok/Sok type I toxin-antitoxin system is encoded in chromosomes and plasmids of multiple Gram-negative bacteria. Current knowledge about hok/Sok regulation, toxicity and function originates mostly from the system present in the R1 plasmid, alongside a handful of homologs. The recent expansion of bacterial genome sequences calls for an updated analysis of hok/Sok diversity and distribution. Here, we used protein and DNA sequences as well as RNA structure to search for Hok homologs. Hok was detected in almost 80% of the available Enterobacteriaceae genomes and, more rarely, in three other bacterial families. Similarity, clustering analysis of thousands of Hok homologs unveiled high Hok sequence variability and uncovered clusters of novel homologs. Experimental validation of representative sequences revealed their toxicity, regardless of their localization. After scanning dedicated databases, we observed an enrichment of the hok sequences in prophages and large conjugative plasmids. Finally, we identified horizontal gene transfer events across genera, predominantly mediated by plasmids. Our findings yield a comprehensive and curated catalog of annotated sequences, detailing genomic localization, toxic activity and distribution across diverse bacterial genomes.

bioinformatics↗