Pericyte ablation causes hypoactivity and reactive gliosis in adult mice
Pericytes are contractile cells that enwrap capillaries allowing them to control blood flow, maintain the blood-brain barrier and regulate immune cell trafficking in the CNS. Pericytes are lost or become dysfunctional in neurodegenerative diseases such as Alzheimers disease, stroke, and multiple sclerosis, but their role in health and disease is poorly understood. Our aim was to evaluate blood-brain barrier integrity and glial reactivity, and to assess behavioural phenotypes that emerge following pericyte ablation in adult mice. The delivery of tamoxifen to PDGFR{beta}-CreERT2 :: Rosa26-DTA transgenic mice produced a dose-dependent ablation of pericytes. A single low dose of tamoxifen ablated approximately half of all brain pericytes, and two consecutive daily high doses ablated more than 80% of brain pericytes. To determine whether pericyte ablation could induce a behavioural phenotype, we assessed patterns of voluntary movement, as well as balance and coordination using the open field and beam walk tasks. Mice with [~]50% pericyte loss travelled half the distance and spent half as much time moving in the open field as control mice. Mice with more than 80% pericyte ablation also slipped more frequently in the beam walk task than control mice. In brain cryosections from pericyte-ablated mice, blood vessel structure was unchanged, but lumen area was increased. Pericyte-ablated mice also experienced blood-brain barrier leakage, hypoxia and increased microgliosis and astrogliosis compared to control mice. Our results highlight the importance of pericytes for brain health, as pericyte loss can directly drive brain injury and behavioural alterations in mice.