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Carvajal, T.

Publications and source records attributed to Carvajal, T..

2 recordsLinked to original sources

Using Google Trends to Examine the Spatio-Temporal Incidence and Behavioral Patterns of Dengue Disease: A Case Study in Metropolitan Manila, Philippines

Dengue is a major public health concern and an economic burden in the Philippines. Despite the countrys improved dengue surveillance, it still suffers from various setbacks and therefore needs to be complemented with alternative approaches. Previous studies have demonstrated the potential of internet-based surveillance such as Google Dengue Trends (GDT) in supplementing current epidemiological methods for predicting future dengue outbreaks and patterns. With this, our study aims to assess the temporal relationship of GDT and dengue incidence in Metropolitan Manila from previous years and examine web search behavior of the population towards the disease. The study collated and organized the population statistics and reported dengue cases in Metropolitan Manila from respective government agencies to calculate the spatial and temporal dengue incidence. The relative search volume of the term dengue and top dengue-related search queries in Metropolitan Manila were obtained and organized from the Google trends platform. Data processing of GDT and dengue incidence was performed by conducting an adjustment procedure and subsequently used for correlation and cross-correlation analyses. Moreover, a thematic analysis was employed on the top dengue-related search queries. Results revealed a high temporal relationship between GDT and dengue incidence when either one of the variables is adjusted. Cross-correlation showed that there is delayed effect (1-2 weeks) of GDT to dengue incidence, demonstrating its potential in predicting future dengue outbreaks and patterns in Metropolitan Manila. Thematic analysis of dengue-related search queries indicated 5 categories namely; (a) dengue, (b) sign and symptoms of dengue, (c) treatment and prevention, (d) mosquito and (e) other diseases where the majority of the search queries was signs and symptoms which indicate the health-seeking behavior of the population towards the disease.

epidemiology

DETECTION OF Wolbachia IN FIELD-COLLECTED MOSQUITO VECTOR, Aedes aegypti

It was the impression from past literature that Wolbachia is not naturally found in Ae. aegypti. However, there are have been reports that recently reveals the presence of this endosymbiont in this mosquito vector. With this, our study presents additional support of Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using Wolbachiaspecific 16S rDNA and its surface protein (wsp) makers under optimized PCR conditions. From a total of 672 Ae. aegpyti adult mosquito samples collected in Metropolitan Manila, Philippines, 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be Wolbachia infected using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A or B while majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples revealed to show positive amplifications in both markers and 69.0% showed congruence in supergroup identification (supergroup B). Our findings illustrate that the infection status of Wolbachia in Ae. aegypti may appear common than previously recognized.

microbiology