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Biology subjects

Carichino, L.

Publications and source records attributed to Carichino, L..

2 recordsLinked to original sources

Quantitative Model of the Ocular Immune Response during Seasonal Allergic Conjunctivitis

Allergic conjunctivitis is an inflammation of the conjunctiva caused by allergen; it is common disorder affecting up to 40% of the population. In this work, we study seasonal allergic conjunctivitis (SAC), also called "hay fever eyes", which is caused by exposure to airborne pollens. We develop a mathematical model quantifying the ocular immune system response to the allergens. First, we present a simplified qualitative description of the immunopathogenesis of SAC. Then, we express each chosen immunopathological mechanism mathematically to construct a system of thirty-one ordinary differential equations. We compare summary statistics of the predicted observable immune signals to experimental measurements and find our model captures key qualitative features of SAC progression. We then compare our predicted time series of histamine concentration to symptom scores and find a strong correlation suggesting the model predicts relevant clinically trends. Next, we calibrate the model through multi-step process. We find the most influential parameters are the production and depletion rates of IL-4, and the production rates of IL-5 and IL-8. These cytokines are targeted in treatments for asthma, atopic dermatitis, and severe eosinophilic associated disorder and suggest potential therapeutic targets for SAC. Our calibrated model mimics most of the summary statistics of the experimentally observable immune signals with discrepancies for IL-5 and IL-13 indicating that additional immunopathological mechanisms could be important.

pathology↗

A meta-analysis of in vitro release of hydrophilictherapeutics from contact lenses using mathematicalmodeling

A meta-analysis was conducted to study the in vitro release of hydrophilic therapeutics from contact lenses. Fifty-two experiments were studied that measure the cumulative release of therapeutics from (mostly) commercial contact lenses placed in a vial. A mathematical model and a parameter fitting algorithm is presented to estimate the diffusion coefficient (D) and 50% therapeutic release time (T50) of all the experimental lens-therapeutic combinations. The mathematical framework was validated against previous studies. Statistical methods were used to analyze the relationships between lens materials, therapeutic properties, and predicted parameter values (D and T50). It was found that lens water content directly and moderately influences the estimated diffusion coefficient. More specifically, the median diffusivity of silicone hydrogel (SH) contact lenses was statistically different from conventional hydrogel (CH) lenses. Other lens and therapeutic properties dependencies on diffusivity were complex with special cases studied to elicit dependencies. A predictive tool was constructed to estimate the logarithm of 50% therapeutic release time, log(T50), given the lens water content and the therapeutic molecular volume and density. The statistical model explained 64% of the variability of the log(T50) and can be used in the preliminary stages of contact lens drug delivery development.

pharmacology and toxicology↗