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Capizzi, M.

Publications and source records attributed to Capizzi, M..

2 recordsLinked to original sources

Rescuing unseen stimuli through alerting retro-cues

AO_SCPLOWBSTRACTC_SCPLOWCan alerting retro-cues improve the conscious perception of stimuli that would otherwise remain unseen? While pre-stimulus alerting is known to facilitate conscious access, the effects of retro-cues remain ambiguous due to methodological confounds in existing literature. Specifically, most studies finding retro-cue benefits have relied on spatial features (such as lateralized targets or cues), which confound alerting with spatial selection. Our design addresses this gap by employing central visual targets and non-lateralized auditory cues, thereby isolating the temporal boost of phasic alerting from spatial orienting. Across four experiments, participants reported the presence and orientation of a central Gabor patch presented at near-threshold ([~]50% detection)) or higher visibility ([~]75% detection) levels. An auditory alerting tone was presented prior to, simultaneously with, or after the Gabor, at various short and long stimulus onset asynchronies, with both short and long temporal ranges. Results consistently showed that pre-stimulus and simultaneous cues significantly enhanced conscious perception, increasing both seen rates and (in some experiments) perceptual sensitivity. Crucially, the presence of retro-cue benefits was observed at higher stimulus visibility, but not at near-threshold, suggesting a potential influence of target visibility on retro-cue efficacy. When targets were near-threshold, retro-cues did not reliably improve conscious perception, whereas tones presented 200 ms after target offset significantly increased the proportion of seen reports when target visibility was increased . These findings indicate that nonspatial auditory retrocues can modulate conscious report when the underlying sensory trace is sufficiently robust. More broadly, they show that the timing of conscious access can be influenced by late alerting signals even in the absence of spatial information, providing a behavioural platform to refine models of how alerting and evidence strength interact in conscious perception.

neuroscience↗

Huntingtin bundles and changes the local proteome of actin filaments in neurons

Huntingtin (HTT) is a large protein whose best-known function being the facilitation of intracellular dynamics along the microtubule network by scaffolding molecular motors complexes. Our recent finding that the defective axonal growth in HD was due to altered growth cone architecture led us to ask whether HTT also influences the cytoskeleton itself. In developing neurons, we found that a large proportion of HTT associates with F-actin in growth cones. Using cell free system and purified recombinant proteins, we observed that HTT binds directly filamentous actin (F-actin) and organizes filaments into bundles. Transmission electron microscopy shows that HTT dimers crosslink adjacent filaments 20 nm apart. We also provide evidence that HTT binding on F-actin modulates the association of other proteins to this cytoskeleton. Notably, HTT limits the association of the growth cone protein Drebrin1 with F-actin. HTT depletion leads to abnormal cytoskeletal organization, localization of Drebrin1 in growth cones, and axonal growth. HTT therefore serves a scaffolding function for the cytoskeleton itself, what might be relevant for HD pathophysiology.

cell biology↗