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Bokil, N. V.

Publications and source records attributed to Bokil, N. V..

2 recordsLinked to original sources

Track Display Jockey (trackDJ): a user-friendly R package for visualization of epigenomic data

BackgroundVisualization of epigenomic data such as coverage tracks, peak calls, and chromatin interactions is a critical task in genomic data analysis. Although genome browsers such as the Integrative Genomics Viewer (IGV) and the UCSC Genome Browser permit user-friendly exploration of genomic tracks, they are not optimized for fully programmatic and reproducible generation of publication-quality figures. In contrast, existing programmatic tools lack a user-friendly interface and require extensive configuration. ResultsWe present trackDJ (Track Display Jockey), an R package for visualization of epigenomic data. trackDJ prioritizes usability by favoring convention over configuration; it provides high-level plotting functions with sensible defaults, allowing users with minimal programming experience to generate clear, publication-quality figures with relatively little coding. Within a unified plotting framework, users can stack and align multiple data types, including coverage tracks, peak annotations, chromatin loops, and gene annotations. trackDJ allows users to select plotted genomic regions by coordinates or by gene name, enabling rapid visualization without knowledge of precise locus boundaries. ConclusionstrackDJ provides a user-friendly and reproducible alternative to interactive genome browsers for epigenomic visualization, filling a critical gap in currently available epigenomics toolkits. By enabling scripted generation of clean, customizable genomic illustrations, trackDJ integrates naturally into R-based analysis workflows to streamline the creation of publication-quality figures.

bioinformatics↗

The human Y and inactive X chromosomes similarly modulate autosomal gene expression

Somatic cells of human males and females have 45 chromosomes in common, including the "active" X chromosome. In males the 46th chromosome is a Y; in females it is an "inactive" X (Xi). Through linear modeling of autosomal gene expression in cells from individuals with zero to three Xi and zero to four Y chromosomes, we found that Xi and Y impact autosomal expression broadly and with remarkably similar effects. Studying sex-chromosome structural anomalies, promoters of Xi- and Y-responsive genes, and CRISPR inhibition, we traced part of this shared effect to homologous transcription factors - ZFX and ZFY - encoded by Chr X and Y. This demonstrates sex-shared mechanisms by which Xi and Y modulate autosomal expression. Combined with earlier analyses of sex-linked gene expression, our studies show that 21% of all genes expressed in lymphoblastoid cells or fibroblasts change expression significantly in response to Xi or Y chromosomes.

genetics↗