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Biology subjects

Belic, M.

Publications and source records attributed to Belic, M..

3 recordsLinked to original sources

An AI-ready compositional framework for mechanistic aging research and in silico intervention testing

Aging is a network-level phenomenon, with its hallmarks interacting through dense feedback loops across vastly different timescales. Decades of reductionist research have produced thousands of mechanistic models of narrow subsystems, but no straightforward way to integrate them into one comprehensive system. Consequently, whole-cell and multi-hallmark aging models remain rare, manually constructed, and relatively small, despite broad agreement on their importance. The emergence of autonomous research agents offers a way to distribute this modeling effort across humans and artificial intelligence and thereby greatly accelerate it, but only if the underlying substrate allows agents to readily compose and analyze models with built-in validation checks and modeling guidance, without extensive custom code. We present hallsim, a JAX-native compositional simulation framework designed as the mechanistic substrate for agent-orchestrated research. It provides composability, end-to-end differentiability, GPU execution, and automated analysis and model-selection tools that facilitate composite model construction and fine tuning. The framework makes it easier to propose a mechanistic hypothesis, integrate it into an existing composite, reparametrize it against observed data, and batch-test it across different initial conditions. We demonstrate the co-simulation and in silico perturbation of three independently published kinetic models spanning genomic instability, nutrient sensing, and proteostasis. The models are connected by four edges and calibrated against a public dataset. Additionally, we train a Neural ODE surrogate and compose it alongside the mechanistic modules in a hybrid composite, demonstrating that mechanistic and neural models can function as complementary components of the same system.

systems biology↗

Proteogenomic Profiling Reveals a Distinct Endogenous p16INK4a-Associated Senescence Signature in the Human Ovary.

The tumor suppressor and cell cycle regulator, p16INK4a (p16), has been extensively linked to cellular senescence, and its accumulation can reflect endogenous senescence within ovarian tissue. However, gene and protein signatures associated with p16 have not been well defined in human tissue. We utilized immunohistochemical (IHC) staining for P16 to identify distinct positive (P16+) and negative (P16-) regions within the ovarian cortex and employed the GeoMx Digital Spatial Profiler for simultaneous proteomic and transcriptomic analyses on cortical tissue cores. Differential expression and translation between p16-positive and p16-negative cores identified genes and proteins that are cellular senescence related (e.g., CDKN1A, GADD45B, GADD45G, and MYC) or key regulators of the extracellular matrix (e.g., collagen I, ADAMTS4, and MMP11). Additionally, the transcriptomic signature identified here was significantly enriched for the spatially derived ovarian p16-associated signature, BuckSenOvary, but not for other senescence gene sets. Lastly, given the association between changes to the extracellular matrix in aged ovaries and ovarian cancer, we compared genes upregulated and downregulated in p16-positive regions relative to p16-negative regions against multiple ovarian cancer transcriptomic datasets. These findings provide new insight into the molecular landscape of naturally occurring ovarian senescence and its possible relationship to age-associated disease processes, including cancer development.

cell biology↗

Facial photographs as proxies for inflammatory aging

Systemic chronic inflammation is a major determinant of aging and disease risk, yet current biomarkers such as the Inflammatory Age (iAge) clock and other proteomic assays remain costly, invasive, and poorly scalable. The skin, as both a visible marker and contributor to age-related inflammation ("inflammaging"), offers a potential non-invasive window into this health metric. Here, we developed Healthy Selfie, a digital predictor of iAge, from simple 2D frontal face photographs. We leveraged data from the Edifice Health Pre-Market trial, a clinical study of 750 participants aged 20 to 90 years, using a subset with available iAge measurements paired to facial images, demographic, clinical, and functional data. Facial embeddings from a pretrained deep learning image model were combined with chronological age, sex and other easily obtainable metadata, and mapped to blood-derived iAge using regression within a leave-one-out cross-validation framework. Photo-predicted iAge values were significantly correlated with blood iAge values (r = 0.536) and were used to identify increased iAge acceleration (accuracy 66.39%, sensitivity 65.61%, specificity 67.24%). Validation on an external dataset containing [~]100,000 images showed no significant demographic bias across ethnicities, including Asian, Black, Indian, Middle Eastern, and Latino populations. Beyond iAge, we also showed that facial features could predict blood levels of individual inflammaging proteins (CXCL9, CXCL1, CCL11, TNFSF10, and IFNG). Our findings suggest that ordinary facial photos can provide information on blood inflammation and can be used as a scalable, ultra low-cost tool for assessing biological aging and advancing precision health.

bioinformatics↗