Biology subjects

Bashyal, S.

Publications and source records attributed to Bashyal, S..

2 recordsLinked to original sources

bioRxiv2024

Medicago truncatula CORYNE regulates inflorescence meristem branching, nutrient signaling, and arbuscular mycorrhizal symbiosis

The CLAVATA signaling pathway regulates plant development and plant-environment interactions. CLAVATA signaling consists of mobile, cell-type or environment-specific CLAVATA3/ESR-related (CLE) peptides, which are perceived by a receptor complex consisting of leucine-rich repeat receptor-like kinases such as CLAVATA1 and receptor-like proteins such as CLAVATA2, which often functions with the pseudokinase CORYNE (CRN). CLAVATA signaling has been extensively studied in various plant species for its role in meristem maintenance and in legumes for modulating root interactions with nitrogen-fixing rhizobia. Some signaling proteins involved in development and nodulation, including CLAVATA1, also regulate plant interactions with mutualistic arbuscular mycorrhizal (AM) fungi. However, our knowledge on AM symbiosis regulation by CLAVATA signaling remains limited and only a handful of genetic regulators have been identified. Here we report that Medicago truncatula CRN controls inflorescence meristem branching and negatively regulates root interactions with AM fungi. MtCRN functions partially independently of the AM autoregulation signal MtCLE53. Transcriptomic data revealed that crn roots display signs of perturbed nutrient, symbiosis, and stress signaling, suggesting that MtCRN plays various roles in plant development and interactions with the environment.

plant biology↗

bioRxiv2022

A single Verticillium dahliae effector determines pathogenicity on tomato by targeting auxin response factors

SUMMARYVerticillium dahliae is a xylem-invading fungal pathogen that causes devastating vascular wilt diseases on hundreds of plant hosts, including tomato (Solanum lycopersicum). Although individual V. dahliae strains are typically characterized by their broad host range, differential pathogenicity occurs on nearly all hosts. Currently, the molecular basis underlying such pathogenicity differences remains unknown. We used comparative genomics to identify a single effector gene that specifically occurs in tomato-pathogenic V. dahliae strains and is expressed during tomato colonization. Functional analyses showed that this Tom1 effector governs pathogenicity on tomato, as Tom1 deletion prohibited tomato colonization, while introduction of Tom1 into non-pathogenic V. dahliae or into saprophytic sister species V. tricorpus and V. nubilum resulted in disease. Through proteomics-based approaches, auxin response factors (ARFs) were identified as in planta targets of Tom1. Intriguingly, repression of SlARF2a expression by virus-induced gene silencing fully impaired V. dahliae colonization of tomato, solidifying its role as susceptibility target.Collectively, our findings indicate that a single effector, Tom1, mediates pathogenicity of V. dahliae on tomato by targeting auxin response factors.

plant biology↗