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Biology subjects

Barrera, M.

Publications and source records attributed to Barrera, M..

2 recordsLinked to original sources

Estimating soil mineral nitrogen from data-sparse field experiments using crop model-guided machine learning approach

Sandy soils are susceptible to excessive nitrogen (N) leaching under intensive crop production which is linked with the soils low nutrient holding capacity and high-water infiltration rate. Estimating soil mineral nitrogen (SMN) at the daily time-step is crucial in providing fertilizer recommendations balancing plant nitrogen use efficiency (NUE) and N losses to the environment. Crop models [e.g., Decision Support System for Agrotechnology Transfer (DSSAT)] can simulate the trend of SMN in varied fertilizer rates and timing of application but are unable to replicate its magnitude due to the inability to capture high-water table conditions in a sub-irrigated soil. As an alternative to such physics-based model, time-series deep learning (DL) models based on a long short-term memory (LSTM) are promising in understanding nonlinearity among complex variables. Yet, purely data-driven DL models for crops are difficult to obtain due to the insufficient amount of data available and the excessive costs with producing more data. To address this challenge, a hybrid model (hybrid-LSTM) was developed by leveraging both the DSSAT and LSTM models to estimate daily SMN primarily using daily weather, applied fertilizer rates-timings, and the SMN sparse observations. This study used the observations from field trials conducted between 2010-2014 in Hastings, FL. The first step was to calibrate the DSSAT-SUBSTOR-Potato model to produce reliable SMN of the topsoil for treatments with varied N applied fertilizer rates split among the pre-planting, emergence, and tuber-initiation stages of the potato crop. Thereafter, the hybrid-LSTM model was trained on the calibrated DSSAT simulated SMN time-series and fine-tuned its predictions using the observed SMN to improve DSSAT simulated SMN. The hybrid-LSTM model was then tested on both calibrated and uncalibrated DSSAT SMN simulations where it outperformed the DSSAT model (range of improvement ranged [~]18-30% on comparing the normalized root mean squared error) in providing reliable estimates of SMN across most of the farms and years. This novel hybrid modeling approach could guide stakeholders and farmers to build sustainable N management with improved crop NUE and yield and help in minimizing environmental losses.

physiology↗

Characterisation of type 2 diabetes progression with regulatory network

Type 2 diabetes develops due to beta cell exhaustion with accompanying decrease in insulin secretion, leading to hyperglycemia and eventual damage of nerve, kidney, and eye tissues. It is usually preceded by metabolic alterations related to insulin signaling, inflammatory pathways, or intracellular glucose processing, encompassed as metabolic syndrome. We propose a regulatory network for components of pancreatic-beta cells playing an essential role in the disease. The network interactions are expressed as continuous fuzzy logic propositions. The dynamical modeling of the network allows to portray the disease progression as a transit between steady states associated to health, metabolic syndrome, and diabetes, each state defined by specific expression patterns of the network components. Transitions between equilibrium states are due to altered expression or functional exhaustion associated to modifications of characteristic decay rates of cellular components. This approach let us identify functional modules that may eventually drive the transit from health to diabetes. The analysis reveals that underexpression of protein kinase B and X-box binding protein 1, with concomitant overexpression of lipopolysaccharides and thioredoxin interacting protein are key factors in the transition from health to disease.

systems biology↗