Biology subjects

Barik, S. K.

Publications and source records attributed to Barik, S. K..

4 recordsLinked to original sources

bioRxiv2025

Phytochemical Content and Antimicrobial Activity of Aloe Vera extract of Marathwada Region, India

Aloe vera (Aloe barbadensis) is widely recognized for its therapeutic properties due to its rich phytochemical composition, including proteins, carbohydrates, and steroids. The study aimed to evaluate the efficiency of solvents such as ethanol and methanol for extracting bioactive compounds and to analyse the phytochemical contents of Aloe vera extracts using various biochemical assays. The dinitro salicylic acid test (DNSA) quantified reducing sugars with ethanol extracts yielding 870 mg/ml compared to 450 mg/ml for methanol extracts. The Biuret test for proteins revealed concentrations of 13.8 mg/ml in ethanol extracts and 13.03 mg/ml in methanol extracts. The Barfoeds test indicated carbohydrate concentrations of 960 mg/ml in ethanol extracts. A qualitative test for steroids confirmed their presence in both solvents, with ethanol showing a more intense reaction, suggesting a higher concentration of steroidal compounds. The results establish ethanol as a more efficient solvent for extracting phytochemicals from Aloe vera. Antimicrobial activity test against E. coli demonstrated superior inhibition zones for ethanol extract, highlighting their potential as natural antibacterial agents. The research indicates the significance of solvent selection in optimizing the therapeutic utility of Aloe vera. It provides a foundation for further exploration into its bioactive compounds and mode of action.

plant biology↗

bioRxiv2023

Codon usage bias study of the Vitamin D receptor (VDR) gene of Multiple Sclerosis and Diabetes-1 patients

ObjectiveThe codon bias usage study of the 33 Vitamin D Receptor (VDR) genes of the Multiple sclerosis and Diabetes-1 patients were characterized. MethodsVarious computational tools such as clustal-W, Codon adaptation index calculation (CAI cal), Effective number of codons (ENC), Relative Synonymous Codon Usage, Codon usage frequency, Nucleotide substation rate calculation, Relative codon deoptimization index, Grand average hydropathicity, Sequence Manipulation Suite softwares were used to find out the codon usage pattern of the VDR genes in both group of patients. ResultsThe base compositions, nucleotide substation rates, codon adaptation index, hydrophobic nature of the amino acids of VDR gene of both group of patients were analysed. Conclusion: The analysis of the synonymous codon usage pattern of the genes would helpful in the heterologous expression of the VDR genes leads to codon optimizations in Multiple Sclerosis and Diabetes-1 patients. The codon usage bias analysis of the VDR gene of the Multiple sclerosis and Diabetes-1 patients through computational approach determined the pattern of VDR gene expression and evolution during the acquiring of the disease in the patients.

bioinformatics↗

bioRxiv2021

Comparative evaluation of plasma protein purification and 2-D gel electrophoresis protocols for analysis of HIV-1 infected human plasma proteins

Purification of proteins from human plasma is a herculean task to perform 2-D gel electrophoresis. Human plasma contains nearly 70% albumin and globulin. The removal of such high abundance high molecular weight proteins is very difficult before performing 2-D gel electrophoresis. It becomes more difficult when we intent to investigate in infectious diseases like HIV/AIDS. We tried to the best of our efforts adopting various organic and non-organic based protocols based on various published papers. After failure of these protocols in results of 2-D gel-electrophoresis Aurum serum mini kit (Bio-Rad, USA) was adopted for plasma protein purification for performing 2-D gel electrophoresis. The low-abundance proteins were better resolved by 10% SDS-PAGE in 2-D gel-electrophoresis. Then,we extended the MALDI-TOF/TOF analysis of low-abundance proteins in human plasma by adopting the Aurum serum mini kit (Bio-Rad, USA) for 2-D gel electrophoresis. Thus, we concluded that, depletion of high abundant proteins like albumin and globulin, the use of the Aurum serum mini kit (Bio-Rad, USA) is the protocol of choice to perform the 2-D gel electrophoresis of HIV-1 infected human plasma.

biochemistry↗

bioRxiv2021

Identification and characterization of novel plasma proteins in drug resistant HIV/AIDS patients by SWATH-MS

Background and ObjectivesAcquired immunodeficiency syndrome is one of the most important diseases caused by human immunodeficiency virus. Understanding its molecular pathogenesis is essential to manage the disease at the population level. In this study, a quantitative analysis of plasma proteins was carried out in drug resistant and drug respondent patients using the SWATH-MS. MethodsSequential window acquisition of all theoretical mass spectra (SWATH-MS) is a prime technique to seek the key plasma proteins involved in virus replication and drug metabolism during therapy. ResultsIn total, 204 proteins were identified and quantified, 57 proteins were differentially expressed, 25 proteins were down regulated and 32 proteins were upregulated in drug resistant patients. Six proteins such as complement C4-A, immunoglobulin heavy variable 1-2, carboxylic ester hydrolase, fibulin-1, immunoglobulin lambda constant 7, secreted phosphoprotein 24 were statistically expressed in drug resistant patients compared to the drug respondent patients. Gene ontology study and protein-protein interaction networks were established in six statistically significant differentially expressed proteins of the drug resistant patients. Interpretation and ConclusionOur findings high lights the novel proteins that were differentially expressed in drug resistant patients. A label-free quantitative proteomics method for depleted human plasma samples by SWATH-MS that can be useful in plasma proteomics research in any biological system.

biochemistry↗