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Biology subjects

Arce-McShane, F.

Publications and source records attributed to Arce-McShane, F..

3 recordsLinked to original sources

An Integrated Approach to Tracking Mandibular Position Relative to Incisal and Condylar Envelopes of Motion During Intraoral Clinical Procedures: A New Look at TMJ Movements

Current methods for tracking temporomandibular joint (TMJ) movements are difficult to perform during dental procedures. Yet precise and accurate quantification of mandibular movements is critical for understanding temporomandibular biomechanics and how it may contribute to temporomandibular dysfunction. This is particularly relevant to clinical procedures that might move the mandible near or beyond its functional range of motion. We present a novel approach that integrates cone-beam computed tomography, optical intra-oral scans, and six degree-of-freedom electromagnetic sensor data to quantify mandibular movements. This method employs rigid body transformations to generate subject-specific three-dimensional (3D) envelopes of motion and assess whether incisal and condylar landmarks remain within their functional 3D envelopes of motion. We demonstrate the clinical utility of this approach through simulated mandibular poses presented relative to the limits of incisal and condylar envelopes created from that individuals voluntary border movements. Our findings reveal that condylar or incisal points in simulated mandibular poses are located beyond their normal motion envelopes, highlighting the importance of simultaneous monitoring of incisal and condylar landmarks. This methodology provides a clinically relevant tool for understanding temporomandibular biomechanics and it has the potential to signal clinicians when jaw movements during dental and oral surgical procedures approach or exceed the jaws functional range of motion and thereby could prevent adverse effects on the TMJ.

bioengineering↗

Multiple regions of primate orofacial sensorimotor cortex encode bite force and gape

The precise control of bite force and gape is vital for effective breakdown and manipulation of food inside the oral cavity during feeding. Yet the role of the orofacial sensorimotor cortex (OSMcx) in the control of bite force and gape is still largely unknown. The aim of this study was to elucidate how individual neurons and populations of neurons in multiple regions of OSMcx differentially encode bite force and gape when subjects (Macaca mulatta) generated different levels of bite force at varying gapes. We examined neuronal activity recorded simultaneously from three microelectrode arrays implanted chronically in the primary motor (MIo), primary somatosensory (SIo), and cortical masticatory (CMA) areas of OSMcx. We used generalized linear models to evaluate encoding properties of individual neurons and utilized dimensionality reduction techniques to decompose population activity into components related to specific task parameters. Individual neurons encoded bite force more strongly than gape in all three OSMCx areas although bite force was a better predictor of spiking activity in MIo versus SIo. Population activity differentiated between levels of bite force and gape while preserving task-independent temporal modulation across the behavioral trial. While activation patterns of neuronal populations were comparable across OSMCx areas, the total variance explained by task parameters was context-dependent and differed across areas. These findings suggest that the cortical control of gape may rely on computations at the population level whereas the strong encoding of bite force at the individual neuron level allows for the precise and rapid control of bite force. Significance StatementBiting a piece off an apple requires precise sensorimotor control and coordination of bite force and gape by multiple brain regions. The cortical representations of bite force and gape by individual neurons and large populations of neurons across connected motor and somatosensory areas in orofacial cortex is unknown. Here we showed that bite force was more strongly encoded than gape by individual neurons in primary motor, somatosensory, and cortical masticatory areas. Moreover, bite force was more effectively represented in motor versus somatosensory cortices. At the population level, bite force and gape were distinguishable particularly when gape was randomized from trial-to-trial. The results are important for understanding neurophysiological processes underlying masticatory dysfunctions that may occur in aging, stroke, and Alzheimers disease.

neuroscience↗

Integrating XMALab and DeepLabCut for high-throughput XROMM

Marker tracking is a major bottleneck in studies involving X-ray Reconstruction of Moving Morphology (XROMM). Here, we tested whether DeepLabCut, a new deep learning package built for markerless tracking, could be applied to videoradiographic data to improve data processing throughput. Our novel workflow integrates XMALab, the existing XROMM marker tracking software, and DeepLabCut while retaining each programs utility. XMALab is used for generating training datasets, error correction, and 3D reconstruction, whereas the majority of marker tracking is transferred to DeepLabCut for automatic batch processing. In the two case studies that involved an in vivo behavior, our workflow achieved a 6 to 13-fold increase in data throughput. In the third case study, which involved an acyclic, post mortem manipulation, DeepLabCut struggled to generalize to the range of novel poses and did not surpass the throughput of XMALab alone. Deployed in the proper context, this new workflow facilitates large scale XROMM studies that were previously precluded by software constraints.

animal behavior and cognition↗