Biology subjects

Amini, E.

Publications and source records attributed to Amini, E..

3 recordsLinked to original sources

bioRxiv2026

Effectiveness of Cold Atmospheric Plasma on Staphylococcus Aureus Colonies on Living Animal Tissue Surface

Staphylococcus aureus (S. aureus) is the most common pathogen associated with skin infections worldwide. Significant efforts have been made to identify and develop innovative therapeutic strategies against S. aureus as alternatives to conventional antibiotics. Physical plasma has a broad range of potential uses, with non-destructive disinfection being one of its earliest applications. Although the literature emphasizes the antibacterial properties of cold atmospheric plasma (CAP), the effect of plasma on S. aureus on damaged skin susceptible to S. aureus invasion through the itch-scratch cycle has not been studied to date. Thus, we examined the effectiveness of CAP treatment on S. aureus bacteria in atopic dermatitis lesions using floating electrode dielectric barrier discharge devices, as well as helium and argon plasma jets. Heat distribution on the skin target, ultraviolet C radiation, and ozone generation of plasma jets for the operator of plasma sources were evaluated. Microbial tests confirmed the presence of S. aureus on the lesions of the groups before treatment. The groups exposed to plasma treatment showed a notable reduction in bacterial population compared to the model group (p<0.05). Furthermore, our investigation indicated that plasma treatment reduced pruritus behavior. The findings suggest that cold atmospheric plasma treatment may potentially target skin infections caused by S. aureus in addition to conventional therapies.

microbiology↗

bioRxiv2023

Timed receptor tyrosine kinase signaling couples the central and a peripheral circadian clock in Drosophila

Circadian clocks impose daily periodicities to behavior, physiology, and metabolism. This control is mediated by a central clock and by peripheral clocks, which are synchronized to provide the organism with a unified time through mechanisms that are not fully understood. Here, we characterized in Drosophila the cellular and molecular mechanisms involved in coupling the central clock and the peripheral clock located in the prothoracic gland (PG), which together control the circadian rhythm of emergence of adult flies. The time signal from central clock neurons is transmitted via small neuropeptide F (sNPF) to neurons that produce the neuropeptide Prothoracicotropic Hormone (PTTH), which is then translated into circadian oscillations of Ca2+ concentration and daily changes in PTTH levels. Rhythmic PTTH signaling is required at the end of metamorphosis, and transmits time information to the PG by imposing a daily rhythm to the expression of the PTTH receptor tyrosine kinase (RTK), TORSO, and of ERK phosphorylation, a key component of PTTH transduction. In addition to PTTH, we demonstrate that signaling mediated by other RTKs contribute to the rhythmicity of emergence. Interestingly, the ligand to one of these receptors (Pvf2), plays an autocrine role in the PG, which may explain why both central brain and PG clocks are required for the circadian gating of emergence. Our findings show that the coupling between the central and the PG clock is unexpectedly complex and involves several RTKs that act in concert, and could serve as a paradigm to understand how circadian clocks are coordinated. Significance statementCircadian clocks impose daily periodicities to behavior, physiology, and metabolism, and are synchronized to provide the organism with a unified time through mechanisms that are poorly understood. In holometabolous insects, the circadian control of adult emergence depends on the coupling between the central clock and a peripheral clock located in the prothoracic gland (PG). Here we identify the cellular and molecular mechanism that transmits time information from the central clock to the PG clock. This process is unexpectedly complex and involves a number of receptor tyrosine kinases (RTKs). Such a mechanism may add robustness to the coupling between the 2 clocks and serve as a paradigm for understanding how circadian clocks are coordinated.

neuroscience↗

bioRxiv2020

The brain of Cataglyphis ants: neuronal organization and visual projections

Cataglyphis ants are known for their outstanding navigational abilities. They return to their inconspicuous nest after far-reaching foraging trips using path integration, and whenever available, learn and memorize visual features of panoramic sceneries. To achieve this, the ants combine directional visual information from celestial cues and panoramic scenes with distance information from an intrinsic odometer. The largely vision-based navigation in Cataglyphis requires sophisticated neuronal networks to process the broad repertoire of visual stimuli. Although Cataglyphis ants have been subject to many neuroethological studies, little is known about the general neuronal organization of their central brain and the visual pathways beyond major circuits. Here, we provide a comprehensive, three-dimensional neuronal map of synapse-rich neuropils in the brain of Cataglyphis nodus including major connecting fiber systems. In addition, we examined neuronal tracts underlying the processing of visual information in more detail. This study revealed a total of 33 brain neuropils and 30 neuronal fiber tracts including six distinct tracts between the optic lobes and the cerebrum. We also discuss the importance of comparative studies on insect brain architecture for a profound understanding of neuronal networks and their function.

neuroscience↗