Amezcua-Correa, R. | Search bioRxiv

bioRxiv2025

Multi-spectral optoacoustic microscopy driven by gas-filled hollow-core fiber laser pulses

Multi-spectral optoacoustic microscopy (MS-OAM) requires high-performance light sources capable of delivering multiple intense spectral lines precisely matched to the absorption characteristics of selected biomolecules. We present a gas-filled anti-resonant hollow-core fiber (ARHCF) laser source optimized for near-infrared (NIR) MS-OAM. The hydrogen (H2)-filled ARHCF laser emits multiple spectral lines with high pulse energy and narrow linewidths (<0.1 nm) across a broad spectral range ([~]1100 nm to [~]2200 nm). Several Raman laser lines were generated to overlap with key biomolecular absorption bands, including lipids (1210 nm and 1700 nm), collagen ([~]1540 nm), and water ([~]1400 nm and [~]1900 nm). We demonstrate the systems performance by mapping absorbers in the first and second overtone regions of hair, pig tissue, and collagen samples. This work aims to bring the gas-filled fiber technology in MS-OAM applications and paves the way for high-resolution, label-free bio-imaging across extended infrared and ultraviolet regimes.

biophysics↗

bioRxiv2024

Mid-infrared photoacoustic brain imaging enabled by cascaded gas-filled hollow-core fiber lasers

SignificanceExtending the photoacoustic microscopy (PAM) into the mid-infrared (MIR) molecular fingerprint region constitutes a promising route towards label-free imaging of biological molecular structures. Realizing this objective requires a high-energy nano-second MIR laser source. However, existing MIR laser technologies are limited to either low pulse energy or free-space structure which is sensitive to environmental conditions. Fiber lasers are promising technologies for PAM for their potential of offering both high pulse energy and robust performance against environmental conditions. However, MIR high energy fiber laser has not yet been used for PAM because it is still at the infant research stage. AimWe aim to employ the emerging gas-filled anti-resonant hollow-core fiber (ARHCF) laser technology for MIR-PAM for the purpose of imaging myelin-rich regions in a mouse brain. ApproachThis laser source is developed with a [~]2.75 J high-pulse-energy nano-second laser at 3.4 m, targeting the main absorption band of myelin sheaths, the primary chemical component of axons in the central nervous system. The laser mechanism relies on two-orders gas-induced vibrational stimulated Raman scattering (SRS) for nonlinear wavelength conversion, starting from a 1060 nm pump laser to 1409 nm through the 1st order Stokes generation in the nitrogen-filled 1st stage ARHCF, then, from 1409 nm to 3.4 m through the 2nd stage hydrogen-filled ARHCF. ResultsThe developed Raman laser was used for the first time for transmission-mode MIR-PAM of mouse brain regions containing rich myelin structures. ConclusionsThis work pioneers the potential use of high-energy and nano-second gas-filled ARHCF laser source to MIR-PAM, with a first attempt to report this kind of fiber laser source for PAM of lipid-rich myelin regions in a mouse brain. The proposed ARHCF laser technology is also expected to generate high-energy pulses at the ultraviolet (UV) region, which can significantly improve the lateral resolution of the PAM.

neuroscience↗