Biology subjects

Akanbi, D. A.

Publications and source records attributed to Akanbi, D. A..

2 recordsLinked to original sources

bioRxiv2026

DIRECTION-AWARE INTEGRATED BIOINFORMATICS ANALYSIS REVEALS CONCORDANT AND DISCORDANT MOLECULAR SIGNATURES LINKING TYPE 2 DIABETES MELLITUS AND POLYCYSTIC OVARY SYNDROME

Abstract Polycystic ovarian syndrome and Type 2 diabetes mellitus are complex multivariate diseases that share well-established clinical pathophysiology, yet the molecular basis of their overlap is unknown. Prior bioinformatics studies have identified common differentially expressed genes between these two conditions. However, they have not accounted for the directionality of the gene expression changes observed, potentially obscuring any biological distinctions they may have. This study applied a direction-aware approach to classify molecular signatures between PCOS and T2DM as concordant or discordant. Transcriptomic analysis of GEO datasets GSE138518 (ovarian granulosa tissue, PCOS) and GSE25724 (pancreatic islet tissue, T2DM) identified 225 and 1,302 DEGs, respectively. Venn diagram analysis showed that only three genes (SLC6A8, RGS4, and SORL1) were shared between PCOS and T2DM from the total of 1,527 genes, and all 3 genes were regulated in opposing directions. Disease gene retrieval from the Comparative Toxicogenomics Database, Online Mendelian Inheritance in Man database, and GeneCards showed 214 shared disease-associated genes, with 311 genes unique to PCOS and 496 genes unique to T2DM. Protein-protein interaction construction using STRING (version 12.0) identified 70 interacting nodes. CytoHubba analysis across 6 scoring methods identified 18 high-confidence hub genes, including INS, BCL2, MTOR, LEP, MFN2, and PIK3CD. Functional enrichment analysis identified biological processes including immune activation, apoptosis, and insulin signaling, which were confirmed as the main pathways in KEGG pathway analysis. These findings show that while PCOS and T2DM share limited DEGs, they share common pathogenic pathways.

bioinformatics↗

bioRxiv2025

The Neuroprotective Effects of Ocimum gratissimum-Supplemented Diet on Scopolamine-Induced Memory Impairment in Mice Model of Alzheimer's Disease

BackgroundCognitive decline is a hallmark of Alzheimers disease, a progressive neurodegenerative illness primarily caused by the buildup of amyloid plaque, which is brought on by oxidative stress and neuroinflammation. Currently, therapeutic agents are focused on addressing clinical symptoms with associated side effects. This study aims to explore the neuroprotective potential of Ocimum gratissimum, a plant known for its richness in bioactive compounds. By investigating its effects on cognitive health, this study addresses a significant gap in the literature regarding dietary interventions for Alzheimers disease. MethodsThirty-six animals were divided into six groups of six mice each: the control group received distilled water intraperitoneally; the scopolamine group received only scopolamine (1 mg/kg i.p.); the three test groups were fed 5%, 10%, and 20% Ocimum gratissimum-supplemented diets while also receiving scopolamine (1 mg/kg i.p.); and the positive control group received Donepezil (5 mg/kg) followed by the injection of scopolamine (1 mg/kg i.p.). Donepezil was administered orally 30 minutes before the scopolamine injection. All treatments were administered daily for 14 consecutive days. ResultsThe supplemented diet groups showed significantly improved spatial memory and navigation compared to the scopolamine-only group. Biochemical analyses revealed that O. gratissimum mitigated scopolamine-induced oxidative stress and neuroinflammation, with marked improvements in antioxidant enzyme levels, reduced lipid peroxidation, and modulation of pro-inflammatory cytokines. ConclusionDietary intervention using Ocimum gratissimum leaf was able to improve spatial memory and protect against memory impairment, suggesting its potential as a neuroprotective agent against Alzheimers disease-like pathology.

neuroscience↗