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Biology subjects

Ahrens, A.

Publications and source records attributed to Ahrens, A..

7 recordsLinked to original sources

Asymmetric listening conditions perturb verbal and nonverbal coordination dynamics during conversation

Human conversation depends on continuous coordination across verbal and nonverbal behaviour. Yet this coordination may become perturbed when one person experiences greater listening difficulty. Previous nonverbal joint action studies show that unequal task demands between partners can lead to complementary leader-follower dynamics, but whether similar patterns emerge during natural conversation remains unclear. We investigated how unequal listening conditions affect verbal and nonverbal coordination in 40 same-gender stranger dyads during three 10-minute face-to-face conversations. Each dyad participated in noise-free and asymmetric noise conditions, with one person exposed to speech-shaped noise at a time. Asymmetric noise reduced conversational flow and enjoyment, and shifted the balance of verbal contributions: noise-exposed participants took longer turns and contributed more speaking time, while their noise-free partners produced more backchannels. Interpersonal head-movement coordination became temporally asymmetric under noise, with movements of the noise-exposed participant more often preceding those of their partner. In contrast, interpersonal hand-movement coordination did not differ between conditions. Within individuals, hand coordination was stronger when participants themselves experienced noise than when their partner experienced noise. These findings demonstrate that perturbing one partner's auditory access affects coordination within and between individuals, revealing how unequal interaction demands produce different coordination patterns across verbal and nonverbal behaviour.

physiology↗

SBT-272 improves TDP-43 pathology in the ALS motor cortex by modulating mitochondrial integrity, motility, and function

Mitochondrial defects are one of the common underlying causes of neuronal vulnerability in motor neuron diseases, such as amyotrophic lateral sclerosis (ALS), and TDP-43 pathology is the most common proteinopathy in ALS. Disrupted inner mitochondrial membrane (IMM) reported in the upper motor neurons (UMNs) of ALS patients with TDP-43 pathology is recapitulated in the UMNs of well-characterized mutant hTDP-43 mouse models of ALS. The construct validity, such as common cellular pathology in mice and human, offers a unique opportunity to test treatment strategies that may translate. SBT-272 is a well-tolerated brain-penetrant small molecule that stabilizes cardiolipin, a phospholipid found in IMM, thereby restoring mitochondrial structure and respiratory function. We investigated whether SBT-272 can improve IMM structure and health in UMNs diseased with TDP-43 pathology in our well-characterized UMN reporter line for ALS. We found that SBT-272 significantly improved mitochondrial structural integrity and restored mitochondrial motility and function. This led to improved health of diseased UMNs in vitro. In comparison to edaravone and AMX0035, SBT-272 appeared more effective in restoring health of diseased UMNs. Chronic treatment of SBT-272 for sixty days starting at an early symptomatic stage of the disease in vivo led to a reduction in astrogliosis, microgliosis, and retention of UMN degeneration in the ALS motor cortex. Our results underscore the therapeutic potential of SBT-272, especially within the context of TDP-43 pathology and mitochondrial dysfunction. HighlightsO_LIEarly and progressive upper motor neuron (UMN) degeneration defines ALS pathology C_LIO_LIMitochondrial defects are prominent and common in UMNs with TDP-43 pathology C_LIO_LISBT-272 treatment improves mitochondrial stability, mobility and function C_LIO_LISBT-272 treatment reduces astrogliosis, microgliosis and improves UMN health C_LI

neuroscience↗

Auditory spatial analysis in reverberant audio-visual multi-talker environments with congruent and incongruent visual room information

In multi-talker situation, listeners have the challenge to identify a target speech source out of a mixture of interfering background noises. In the current study it was investigate how listeners analyze audio-visual scenes with varying complexity in terms of number of talkers and reverberation. Furthermore, the visual information of the room was either coherent with the acoustic room or incoherent. The listeners task was to locate an ongoing speech source in a mixture of other speech sources. The 3D audio-visual scenarios were presented using a loudspeaker array and virtual reality glasses. It was shown that room reverberation as well as the number of talkers in a scene influence the ability to analyze an auditory scene in terms of accuracy and response time. Incongruent visual information of the room did not affect this ability. When few talkers were presented simultaneously, listeners were able to quickly and accurately detect a target talker even in adverse room acoustical conditions. Reverberation started to affect the response time when four or more talkers were presented. The number of talkers became a significant factor for five or more simultaneous talkers.

neuroscience↗

Speech intelligibility with various head-related transfer functions: A computational modeling approach

Speech intelligibility is known to be affected by the relative spatial position between target and interferers. The benefit of a spatial separation is, along with other factors, related to the head-related transfer function (HRTF). The HRTF is individually different and thus, the cues that improve speech intelligibility might also be different. In the current study an auditory model was employed to predict speech intelligibility with a variety of HRTFs. The predicted speech intelligibility was found to vary across HRTFs. Thus, individual listeners might have different access to cues that are important for speech intelligibility.

neuroscience↗

Perceptual weighting of binaural lateralization cues across frequency bands

The auditory system uses interaural time- and level differences (ITD and ILD) as cues to localize and lateralize sounds. The availability of ITDs and ILDs in the auditory system is limited by neural phase-locking and by the head size, respectively. Although the frequency-specific limitations are well known, the relative contribution of ITDs and ILDs in individual frequency bands in broad-band stimuli are unknown. To determine these relative contributions, or spectral weights, listeners were asked to judge the lateralization of stimuli consisting of eleven simultaneously presented 1-ERB-wide noise bands centered between 442 Hz and 5544 Hz and separated by 1-ERB-wide gaps. Interaural disparities were applied to each noise band and were roved independently on every trial. The weights were obtained using a multiple linear regression analysis. In a second experiment the effect of temporal context on the spectral weights was investigated. Ten of the noise bands were used as pre- and postcursors and listeners were asked to lateralize the stimuli. Results show that only the lowest- or highest frequency band received highest weight for ITD and ILD, respectively. Temporal context led to significantly enhanced weights given to the band without the pre- and postcursor. The weight enhancement could only be observed at low frequencies, when determined with ITD cues and for low and high frequencies for ILDs. Hence, the auditory system seems to be able to change the spectral weighting of binaural information depending on the information content. PACS43.64.-q {middle dot} 43.64.+r {middle dot} 87.19.lt {middle dot} 43.66.-x {middle dot} 43.66.+y {middle dot} 43.64.Bt

neuroscience↗

Novel engraftment and T cell differentiation of human hematopoietic cells in Art-/- IL2RG-/ SCID pigs

Pigs with severe combined immunodeficiency (SCID) are an emerging biomedical animal model. Swine are anatomically and physiologically more similar to humans than mice, making them an invaluable tool for preclinical regenerative medicine and cancer research. One essential step in further developing this model is the immunological humanization of SCID pigs. In this work we have generated T- B- NK- SCID pigs through site directed CRISPR/Cas9 mutagenesis of IL2RG within a naturally occurring DCLRE1C (Artemis)-/- genetic background. We confirmed Art-/- IL2RG-/Y pigs lacked T, B, and NK cells in both peripheral blood and lymphoid tissues. Additionally, we and successfully performed a bone marrow transplant on one Art-/- IL2RG-/Y male SCID pig with a bone marrow from a complete swine leukocyte antigen (SLA) matched donor without conditioning to reconstitute porcine T and NK cells. Next, we performed in utero injections of cultured human CD34+ selected cord blood cells into the fetal Art-/- IL2RG-/Y SCID pigs. At birth, human CD45+ CD3{varepsilon}+ cells were detected in peripheral blood of in utero injected SCID piglets. Human leukocytes were also detected within the bone marrow, spleen, liver, thymus, and mesenteric lymph nodes of these animals. Taken together, we describe critical steps forwards the development of an immunologically humanized SCID pig model.\n\nOne sentence summaryWe have generated a T- B- NK- SCID pig model through site directed mutagenesis of IL2RG in a naturally occurring Artemis null background and show successful engraftment of human T and B cells in blood and lymphoid organs after in utero injection of human hematopoietic stem cells.

immunology↗

Sound source localization with varying amount of visual information in virtual reality

To achieve accurate spatial auditory perception, subjects typically require personal head-related transfer functions (HRTFs) and the freedom for head movements. Loudspeaker-based virtual sound environments allow for realism without individualized measurements. To study audio-visual perception in realistic environments, the combination of spatially tracked head mounted displays (HMDs), also known as virtual reality glasses, and virtual sound environments may be valuable. However, HMDs were recently shown to affect the subjects HRTFs and thus might influence sound localization performance. Furthermore, due to limitations of the reproduction of visual information on the HMD, audio-visual perception might be influenced. Here, a sound localization experiment was conducted both with and without an HMD and with a varying amount of visual information provided to the subjects. Furthermore, interaural time and level difference errors (ITDs and ILDs) as well as spectral perturbations induced by the HMD were analyzed and compared to the perceptual localization data. The results showed a reduction of the localization accuracy when the subjects were wearing an HMD and when they were blindfolded. The HMD-induced error in azimuth localization was found to be larger in the left than in the right hemisphere. Thus, the errors in ITD and ILD can only partly account for the perceptual differences. When visual information of the limited set of source locations was provided, the localization error induced by the HMD was found to be negligible. Presenting visual information of hand-location, room dimensions, source locations and pointing feedback on the HMD revealed similar effects as previously shown in real environments.

neuroscience↗