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Aguirre, B.

Publications and source records attributed to Aguirre, B..

2 recordsLinked to original sources

Simulating atmospheric drought: Silica gel packets dehumidify mesocosm microclimates

O_LIAs global temperatures rise, droughts are becoming more frequent and severe. To predict how drought might affect plant communities, ecologists have traditionally designed experiments with controlled watering regimes and rainout shelters. Both treatments have proven effective for simulating soil drought. However, neither are designed to directly modify atmospheric drought. C_LIO_LIHere, we detail the efficacy of a silica gel atmospheric drought treatment in outdoor mesocosms with and without a cooccurring soil drought treatment. At California State University, Los Angeles, we monitored relative humidity (RH), temperature, and vapor pressure deficit (VPD) every 10 minutes for five months in a bare-ground experiment featuring mesocosms treated with soil drought (reduced watering) and/or atmospheric drought (silica packets suspended 12 cm above soil). C_LIO_LIWe found that silica packets dehumidified these microclimates most effectively (-5% RH) when combined with reduced soil water, regardless of the ambient humidity levels of the surrounding air. Further, packets increased microclimate VPD most effectively (+0.4 kPa) when combined with reduced soil water and ambient air temperatures above 20{degrees}C. Finally, packets simulated atmospheric drought most consistently when replaced within three days of deployment. C_LIO_LIOur results demonstrate the use of silica packets as effective dehumidification agents in outdoor drought experiments. We emphasize that incorporating atmospheric drought in existing soil drought experiments can improve our understandings of the ecological impacts of drought. C_LI

ecology↗

Neo-functionalization in Saccharomyces cerevisiae: A Novel Nrg1-Rtg3 chimeric transcriptional modulator is essential to maintain mitochondrial DNA integrity

In Saccharomyces cerevisiae, the transcriptional repressor Nrg1 (Negative Regulator of Glucose-repressed genes) and the b/Zip transcription factor Rtg3 (ReTroGrade regulation) mediate glucose repression and mitochondria to nucleus signaling, respectively. Here we show a novel function for these two proteins, in which alanine promotes the formation of a chimeric Nrg1/Rtg3 regulator that represses the ALT2 gene (encoding an alanine transaminase paralogue of unknown function) expression. A NRG1/NRG2 paralogous pair, resulting from a post-wide genome, small scale duplication event, is extant in the Saccharomyces genus. Neo-functionalization of only one paralogue resulted in Nrg1, able to interact with Rtg3. Either nrg1{Delta} or rtg3{Delta} single mutant strains are unable to utilize ethanol and show a typical petite (small) phenotype on glucose. Neither of the WT genes complemented the petite phenotype, suggesting irreversible mitochondrial DNA damage in these mutants. Neither nrg1{Delta} nor rtg3{Delta} mutant strains express genes encoded by any of five polycistronic units transcribed from mitochondrial DNA in S. cerevisiae. This, and the direct measure of the mitochondrial DNA gene complement confirms that irreversible damage of the mitochondrial DNA occurred in both mutant strains and is consistent with an essential role of the chimeric Nrg1/Rtg3 regulator in mitochondrial DNA maintenance.

microbiology↗