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Biology subjects

Adrover, M.

Publications and source records attributed to Adrover, M..

4 recordsLinked to original sources

Methylglyoxal-derived glycation tunes nuclear viscoelasticity

Reactive carbonyl metabolites such as methylglyoxal (MGO) accumulate in diabetes, aging, and metabolic disease, conditions frequently associated with pathological stiffening of human cells and tissues. While glycation of extracellular matrices and cytoskeletal proteins is known to contribute to these mechanical changes, whether the nucleus itself is a direct mechanical target of carbonyl stress remains unknown. Here, by combining advanced atomic force microscopy-based nanomechanical probing with correlative confocal imaging, we show that MGO-derived glycation directly remodels isolated cell nuclei, increasing stiffness, reducing fluidity, and restricting intranuclear deformation propagation. MGO-derived adducts accumulate prominently at the nuclear envelope, and perturbation of lamin A/C and chromatin state reveals that both structures contribute to glycation-induced stiffening. In cells, chronic MGO exposure induces nucleus-intrinsic stiffening and biases YAP nuclear localization in a substrate-stiffness-dependent manner, linking nuclear glycation to altered mechanosensitive regulation. In vivo, cardiac nuclear stiffening is only observed along with increased immunodetected MGO-adduct burden, while nuclear fluidity remains largely preserved. Together, these findings establish nonenzymatic glycation as a metabolic mechanism that remodels nuclear mechanics and alters cellular mechanosensing, revealing a previously unrecognized physical consequence of carbonyl stress.

biophysics↗

Pathological stiffening by crosslinking glycation of titin

Heterogeneous, non-enzymatic glycation chemistry triggered by sugar-derived metabolites is typical of diseases that also entail pathological stiffening of cells, such as diabetes and age-related disorders. However, the mechanisms responsible for cell stiffening and the role of glycated biomolecules remain largely unexplored. Here, we show that glycation of cardiac titin, a giant intracellular protein scaffolding contractile sarcomeres, is increased in diabetes and leads to rigidification of both the protein and cardiomyocytes. Mechanistically, glycation-induced titin stiffening results from decreased contour length and enhanced folding of otherwise structurally intact protein domains following extensive formation of intramolecular crosslinking advanced glycation end products (AGEs). These stiffening effects outweigh softening contributions by competing, non-crosslinking AGEs. In combination, our work overcomes the intrinsic chemical complexity typical of glycation to uncover crosslinking AGEs as a source of pathological stiffening of cells, which we propose contributes to tissue dysfunction in situations of glycative stress.

biophysics↗

Dysregulated Skeletal Muscle Myosin Super-relaxation in Type II, but Not Type I, Diabetes Mellitus

Disrupted energy balance is critical for the onset and development of Type II diabetes. The exact underlying metabolic mechanisms remain incomplete but skeletal muscle is thought to play an important pathogenic role. As the super-relaxed state of its most abundant protein, myosin, regulates cellular energetics, here, we aimed to investigate whether it is altered in patients with type II diabetes. For that, we used vastus lateralis biopsy specimens (obtained from patients with type II diabetes and matched controls) and run a combination of structural and functional assays consisting of loaded Mant-ATP chase experiments, X-ray diffraction and LC-MS/MS proteomics in isolated muscle fibres. Our studies revealed a greater muscle myosin super-relaxation and decreased cellular ATP demand in patients than controls. Subsequent proteomic analyses indicated that these (mal)adaptations likely originated from remodeled sarcomeric proteins and greater myosin glycation levels in patients than controls. Overall, our findings emphasize a complex molecular dysregulation of myosin super-relaxed state and energy consumption in type II diabetes. Ultimately, pharmacological targeting of myosin could benefit skeletal muscle and whole-body metabolic health through the enhancement of ATP consumption. Significance StatementMyosin super-relaxation, essential for the regulation of skeletal muscle metabolic rate, is disrupted in type II diabetes due to protein hyper-glycation. As a consequence, myosin ATP demand is significantly lowered. Overall, our findings provide a strong rationale for the use of activators of myosin ATPase to enhance basal energy expenditure in type II diabetes.

cell biology↗

cAMP-Fyn signaling in the dorsomedial striatum direct pathway drives excessive alcohol use

Fyn kinase in the dorsomedial striatum (DMS) of rodents plays a central role in mechanisms underlying excessive alcohol intake. The DMS is comprised of medium spiny neurons (MSNs) that project directly (dMSNs) or indirectly (iMSNs) to the substantia nigra. Here, we examined the cell-type specificity of Fyns actions on alcohol use. First, we knocked down Fyn selectively in DMS dMSNs or iMSNs of mice and measured the level of alcohol consumption. We found that downregulation of Fyn in dMSNs, but not in iMSNs, reduces excessive alcohol but not saccharin intake. D1Rs are coupled to Gs/olf, which activate cAMP signaling. To examine whether Fyns actions are mediated through cAMP signaling, DMS dMSNs were infected with GsDREADD, and the activation of Fyn signaling was measured following CNO treatment. We found that remote stimulation of cAMP signaling in DMS dMSNs activates Fyn and promotes the phosphorylation of the Fyn substrate, GluN2B. In contract, remote activation of GsDREADD in DLS dMSNs did not alter Fyn signaling. We then tested whether activation of GsDREADD in DMS dMSNs or iMSNs alters alcohol intake and observed that CNO-dependent activation of GsDREADD in DMS dMSNs but not iMSNs increases alcohol but not saccharin intake. Finally, we examined the contribution of Fyn to GsDREADD-dependent increase in alcohol intake, and found that systemic administration of the Fyn inhibitor, AZD0503 blocks GsDREADD-dependent increase in alcohol consumption. Our results suggest that the cAMP-Fyn axis in the DMS dMSNs is a molecular transducer of mechanisms underlying the development of excessive alcohol consumption.

neuroscience↗