Biology subjects

Adewumi, T.

Publications and source records attributed to Adewumi, T..

4 recordsLinked to original sources

bioRxiv2025

Triple-Negative Breast Cancer Cells Activate Sensory Neurons via TRPV1 to Drive Nerve Outgrowth and Tumor Progression

The tumor microenvironment in triple-negative breast cancer (TNBC) is characterized by increased sensory nerve density, which contributes to cancer progression by promoting migration and metastasis. However, the origin of tumor-innervating nerves and the mechanisms driving sensory innervation into tumors remain poorly understood. Using in vivo retrograde labeling techniques, we show that mammary tumors are associated with an increase in nerves originating from the dorsal root ganglia. Additionally, we find that TNBC cells trigger stress and activation markers and induce neuronal firing in a transient receptor potential anilloid subtype 1 (TRPV1)-dependent manner. In both 2D and 3D cell culture models, TNBC cells promote outgrowth of sensory nerves that is abrogated with Trpv1 knockout. We identified c-Jun and IL-6 as an effector of neurite outgrowth that acts downstream of TRPV1 to promote outgrowth in vitro. Finally, in Trpv1 knockout mice, TNBC tumors exhibit delayed growth and reduced lung metastasis. These findings suggest that a critical role for TRPV1 in tumor-nerve crosstalk, offering a potential target to reduce metastatic disease.

cancer biology↗

bioRxiv2025

Specificity of Drosophila innubila Nudivirus Infection in Drosophila Cell Culture

Viral host range is an important aspect of both viral biology but also the pragmatic issue of producing viral stocks for experimentation. Host range is important both in terms of the species a virus can infect (taxonomy), and the types of host cells the virus can infect (tropism). Nudiviruses are large DNA viruses that infect several arthropods and are generally poorly studied. Several nudiviruses infect Drosophila species including the Drosophila innubila Nudivirus (DiNV). We aimed to identify cell lines that support the replication of DiNV both for the sake of understanding host range, and to develop a system for large scale production of the virus for infection. We utilized cell lines from the focal host, D. innubila, as well as available cell lines from D. virilis and D. melanogaster and inoculated with both a wild-collected pool of "naive" virus and a D. innubila cell culture-adapted isolate. We found that virus from wild caught flies infected cells from the 3 cell lines and replicate its genome, but the passage 1 fluids from these infections were unable to reinfect upon introduction to new cells. In contrast, a cell culture-adapted strain of DiNV infected the same Drosophila cells (though relatively poorly in D. melanogaster cells) and produced infectious progeny that infected new cells. Thus, our cell culture-adapted virus developed the ability to infect broadly and produce infectious virions.

microbiology↗

bioRxiv2022

The Main Themes and Experiences Shared with the Hashtag #BlackintheIvory: A Qualitative Analysis

BackgroundThe movement #BlackintheIvory gave Black academics an opportunity to connect through a social media platform allowing them to share common experiences in the pursuit of higher education. Through the analysis of Twitter posts, using the hashtag #BlackintheIvory, this study investigates the main themes identified among Black scholars in academia and their shared experiences with teaching, mentoring, collegiality, identity, service, and racism. MethodsUsing the Twitter API, we isolated all publicly available tweets, which can include text, images, and links to websites, posted with the hashtag #BlackintheIvory on the Twitter website (www.twitter.com) from the inception of the hashtag in June 2020 to the end of December 2020. To evaluate the tweets, we categorized the tweets inductively. Based on the content of the posts, we identified 6 themes: Teaching, Mentoring, Collegiality, Identity, Service, and Racism. ResultsOur search yielded a total of 12,538 original posts, including tweets between inception in June 2020 to December 2020 from profiles made public (excluding modified tweets and duplicate tweets). We selected and analyzed the top retweeted 2500 tweets, which is 20% (2500/12538) of the total number of downloaded tweets. The greatest percentage of posts were about Teaching (881; 35%), followed by Service (441; 18%) and Racism (414; 17%). The remaining tweets were categorized as Collegiality (388; 15%), Identity (210; 8%), and Mentoring (166; 7%) of the total number of tweets from June-December 2020. ConclusionThe experiences, perspectives, and narratives among the Black diaspora within #BlackintheIvory are not uniform. The commonality exists within the structural systemic racism which impacts Black academics within the ivory tower, this study is a resounding wake-up call for action. FundingThis study was not subject to any funding

scientific communication and education↗

bioRxiv2019

An evaluation of Nephrology Literature for Transparency and Reproducibility Indicators: Cross-sectional Review

BackgroundReproducibility is critical to diagnostic accuracy and treatment implementation. Concurrent with clinical reproducibility, research reproducibility establishes whether the use of identical study materials and methodologies in replication efforts permit researchers to arrive at similar results and conclusions. In this study, we address this gap by evaluating nephrology literature for common indicators of transparent and reproducible research.\n\nMethodsWe searched the National Library of Medicine catalog to identify 36 MEDLINE-indexed, English language nephrology journals. We randomly sampled 300 publications published between January 1, 2014, and December 31, 2018. In a duplicated and blinded fashion, two investigators screened and extracted data from the 300 publications.\n\nResultsOur search yielded 28,835 publications, of which we randomly sampled 300 publications. Of the 300 publications, 152 (50.67%) were publicly available whereas 143 (47.67%) were restricted through paywall and 5 (1.67%) were inaccessible. Of the remaining 295 publications, 123 were excluded because they lack empirical data necessary for reproducibility. Of the 172 publications with empirical data, 43 (25%) reported data availability statements, 4 (2.33%) analysis scripts, 4 (2.33%) links to a protocol, and 10 (5.81%) were pre-registered.\n\nConclusionOur study found that reproducible and transparent research practices are infrequently employed by the nephrology research community. Greater efforts should be made by both funders and journals, two entities that have the greatest ability to influence change. In doing so, an open science culture may eventually become the norm rather than the exception.

bioinformatics↗