Abdelsalam, H. | Search bioRxiv

bioRxiv2019

Mitigative effects of Alpha-lipoic acid for the toxicity of Dimethoate in male rats

BackgroundOrganophosphates are widely used by human nowadays, but these compounds have tremendous negative effect on the man health. So this study aims to use of Alpha-lipoic acid (ALA) to alleviates the negative effects of Dimethoate (DM). MethodsThis study is designed as follows, Thirty adult male Wistar albino rats were utilized, further subdivided into control, DM and DM+ALA groups. Liver and renal cortex sections from all groups were processed for histopathological examination, biochemical estimation of liver function tests, serum Urea, Creatinine, BUN, testosterone and lipid profile were performed. ResultsThis study clarified the improvement effects of ALA against the negative effects of DM where ALA caused a significant recovery of the hepatic (ALT, AST, ALP and total protein) and renal functions by normalizing them in DM + ALA group and to some extent improvement of lipid profile and testosterone levels. Also, ALA restored normal hepatic and renal histomorphology. ConclusionIt is concluded that ALA therapy can ameliorate the negative effects of DM that affect the vital organs as the liver and kidney. Also ALA can reduce the occurrence of atherogensis by reducing the levels of bad cholesterol in the blood. ALA boosts the levels of testosterone so it augments the male sexual characters.

physiology↗

bioRxiv2018

The Influence of Magnetic Fields on Selected Physiological Parameters of Blood and Tissues in Mice

This study aimed to highlight the influence of exposure to different applied magnetic fields (MFs) on SOD, MDA and GSH levels in the liver, LDH and CPK activities in the muscle and {gamma}-aminobutyric acid levels in the brain, as well as some haematological parameters. Adult male albino Swiss mice were divided into 5 equal groups (n = 6), the control group (untreated) and four exposure groups that were exposed to MFs of 20, 40, 60 and 80 Gauss for 5 min/day for 5 days.: Exposure to MFs induced significant decreases in total GSH levels and SOD activity but a significant increase in MDA levels in the liver. By contrast, SMF exposure significantly increased total LDH and total CPK activities in the muscle. The results revealed a significant increase in GABA levels in the brain, as well as decreases in haemoglobin, haematocrit, and red blood cell counts, in addition to platelet counts, after exposure to 20, 40, 60 and 80 Gauss MFs. After exposure to a 40 Gauss MF, the mice showed pathological changes in red blood cells, including changes to the outer membrane of the red blood cells (micronucleus and a serrated edge, with a mild incidence of echinocytes). In the group exposed to a 60 Gauss MF, examination of blood smears clearly showed changes in cell size, with the emergence of abnormal forms, including many areas with no red blood cells (rouleaux formation). With increasing intensity of exposure (80 Gauss), the red blood cells appeared completely different from their natural form and took the form of ovalocytes and bi-micronucleated erythrocytes, which appear in patients with anaemia. MF exposure caused different metabolic and haematological effects, which appeared to be related to the intensity of SMF exposure. The changes in the biochemical parameters of SMF-exposed mice probably reflect hepatic damage and anaemia caused by kidney failure. Further studies are needed to obtain a better understanding of the effects of MF on biological systems.

physiology↗